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KMID : 0351819980390010033
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Kyunpook University Medical Journal
1998 Volume.39 No. 1 p.33 ~ p.42
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Cloning of ZNF-dp gene Which Was Identified by Differential Display of mRNA
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Kim Mun-Kyu
Kim Jeong-Chul
Chung Tai-Ho
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Abstract
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To identify the genes specifically expressed in hair mesenchymal tissues, we used differential display of mRNA by using polymerase chain reaction(DD-PCR) technique in cultured cells of human hair follicular dermal papilla, dermal sheath, and fibroblasts. Among many differentially expressed genes we designated one zinc-finger motif containing gene as ZNF-dp and characterized it.
In northern blot analysis, ZNF-dp showed major 3.8 kb ;and minor 5.8 kb transcripts. The ZNF-dp gene was ubiquitously expressed in a variety of human ti:~ues with a low level. Overlapping nine cDNA clones were isolated from dermal papilla and human prostate cDNA library and we could identified 3,594bp sequences from those clones. Homology se:uch result suggests that it is mouse Zfp-35 homolog. The predicted protein structure of the ZNF-dp gene indicated that it encoded 18 contiguous zinc finger domains of the C2Hz type (Kruppel-type), but it contains frameshifr mutations and a inframe stop codon within the zinc finger motif. Further study of ge.nomic structure is needed to elucidate the significance of this gene.
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KEYWORD
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ZNF-dp, dermal papilla cell, hair follicle
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